Developing paper 'origami' eDNA sensors for real-time public surveillance of invasive species

This project aims to develop low-cost, user-friendly and field-ready environmental DNA (eDNA) sensors on folded (origami) paper to detect freshwater invasive species and other biological or chemical targets in near real time. Providing rapid on-site results to support early detection, management and eradication.

The project will develop:

• A paper-based “origami” eDNA device for rapid, on-site species detection.
• A simple field workflow with clear guidance, training, and quality checks for reliable use.
• Practical toolkit to scale monitoring, with materials for sampling, safety, and data reporting.
• Provide more insights to better understand the biodiversity using our advanced environmental sensing technology.

Progress update

We have developed PCR, qPCR, and LAMP primers for the detection of five invasive freshwater species: the killer shrimp (Dikerogammarus villosus), demon shrimp (Dikerogammarus haemobaphes), Chinese mitten crab (Eriocheir sinensis), North American mink (Neovison vison), and American signal crayfish (Pacifastacus leniusculus). These primers will soon be validated through laboratory testing.

Additionally, we have designed a paper-based origami device to facilitate the collection and enrichment of eDNA from water samples and field testing.

In partnership with the University of Lincoln and UKCEH, we will run field demonstrations to support real-time monitoring of ecosystem biodiversity.